Immune-related gene IL17RA as a diagnostic marker in osteoporosis

Objectives: Bone immune disorders are major contributors to osteoporosis development. This study aims identify potential diagnostic markers and molecular targets for treatment from an immunological perspective. Method: We downloaded dataset GSE56116 the Gene Expression Omnibus database, identified differentially expressed genes (DEGs) between normal groups. Subsequently, immune-related (DEIRGs) were identified, a functional enrichment analysis was performed. A protein-protein interaction network also constructed based on data STRING database hub genes. Following external validation using additional (GSE35959), effective biomarkers confirmed RT-qPCR immunohistochemical (IHC) staining. ROC curves validate values of biomarkers. Finally, ceRNA transcription factor constructed, Ontology Kyoto Encyclopedia Genes Genomes performed explore biological functions these markers. Results: In total, 307 31 DEGs DEIRGs respectively. The revealed that mainly associated with terms positive regulation MAPK cascade, granulocyte chemotaxis, cytokine receptor. protein–protein 10 genes: FGF8 , KL CCL3 FGF4 IL9 FGF9 BMP7 IL17RA IL12RB2 CD40LG . expression level found be significantly high. results showed higher in patients compared group, as evidenced by area under curve Area Under Curve 0.802. Then, we NEAT1 -hsa-miR-128-3p- SNHG1 networks addition ERF - IRF8 POLR2A ERG transcriptional networks. involved development progression regulating local inflammatory processes bone tissue. Conclusion: identifies gene marker perspective, provides insight into its function.